The translational and rotational motion of the micro-spike is implemented using a single axis robot-arm. The biopsied tissue is stained using H&E, and the area of the stained tissue is precisely measured using a vision-based image processing program. Each micro-spike has been experimented to analyze the increment of the extracted tissue volume due to the translational and rotational motion during the penetration and retraction. Results: Various designs of micro-spike are fabricated, which differ in the sharpness of two shanks, the width between two shanks, the size of barb, and the number of barbs. The shanks have protruded barbs on their facing sides, which the length, width and thickness is 0.25 mm, 0.05 mm, and 0.5 mm, respectively. The micro-spike has an advantage in submucosal tumor tissue biopsy, since it consists of two shanks like a fork which have 5 mm in length, 1 mm in width and 0.5 mm in thickness.
Therefore, the proposed method is to determine the suitable translational and rotational velocity during the biopsy operation for increment of extracted tissue, which results in acquisition of a clear histological image. Methods: The amount of extracted tissue volume is mainly affected by the penetration and retraction mechanism during the micro-spike biopsy. Also, we developed a vision-based computing method to precisely measure the tissue volume. In this paper, we present a new spiral-motion micro-spike biopsy mechanism. Therefore, it is necessary to increase the amount of tissue extraction. Although the micro-spike is minimal invasive, the amount of extracted tissue is often insufficient for a valid histological diagnosis. The micro-spike penetrates the gastrointestinal wall and extracts tissue by retaining them between the barbed shanks of the micro-spike. To overcome the disadvantages of conventional biopsy tools, we have reported a micro-spike for a minimally invasive biopsy fabricated by the MEMS technology. Objectives: For biopsies, using the conventional macroscale tools may cause significant discomforts, risks, and injuries to patients. Seungju Han Jaekeun Lee Kangil Kim Sangmin Leeĭepartment of Biomedical Engineering, Kyung Hee University, Yongin, Republic of Korea 002 A Spiral-Motion Biopsy Mechanism for Extraction of Submucosal Tissue with Tissue Volume Vision Processing Method The additional researches on the protection mechanisms are required.Īcknowledgements: This work was supported by the Russian Science Foundation (Grants No. The aim of this study is to verify the quantitative model for pathogens growth prediction in fecal culture that will allow to improve synbiotic activity assessment.Ĭonclusions: The model has some prospects for pathogen growth prediction after pH-factor excluding. Commonly, the fecal cultures are applied in synbiotic researches. Previously, the quantitative model of pathogen growth inhibition by probiotic in co-culture fermentation was developed for synbiotic effect evaluation. Objectives: The efficiency of probiotics can be increased by combining with synergistic prebiotic substances. Mendeleev University of Chemical Technology of Russia, Moscow, Russia Mendeleev University of Chemical Technology of Russia, Moscow, Russia 2Laboratory of Applied Genomics and Crop Breeding, All-Russia Research Institute of Agricultural Biotechnology, Moscow, Russia 3Department of Cybernetics of Chemical Technological Processes, D.
Svetlana Evdokimova 1 Boris Karetkin 1 Mikhail Divashuk 2 Victoria Kuznetsova 2 Elena Guseva 3 Victor Panfilov 1ġDepartment of Biotechnology, D. 001 Study and Modeling of Pathogens Growth Inhibition in Fecal Culture
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